Construct: shRNA TRCN0000173418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027476.1-1473s1c1
DNA Barcode:
GCTGGTAGAAATGTAGACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zdhhc24 (70605)
Gene Description:
zinc finger, DHHC domain containing 24
Transcript:
RefSeq NM_027476.1 (NON-CURRENT)
Match location:
Position 1473 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTAGAAATGTAGACCTT
Hairpin Sequence:
5'-CCGG-GCTGGTAGAAATGTAGACCTT-CTCGAG-AAGGTCTACATTTCTACCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTAGAAATGTAGACCTTCTCGAGAAGGTCTACATTTCTACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTAGAAATGTAGACCTTCTCGAGAAGGTCTACATTTCTACCAGC-3'

Other clones with same target sequence:

(none)