Construct: shRNA TRCN0000173467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022656.1-4589s1c1
DNA Barcode:
GAAGTGTTAAGTCCAGTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nisch (64652)
Gene Description:
nischarin
Transcript:
RefSeq NM_022656.1 (NON-CURRENT)
Match location:
Position 4589 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGTTAAGTCCAGTCCTT
Hairpin Sequence:
5'-CCGG-GAAGTGTTAAGTCCAGTCCTT-CTCGAG-AAGGACTGGACTTAACACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGTTAAGTCCAGTCCTTCTCGAGAAGGACTGGACTTAACACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGTTAAGTCCAGTCCTTCTCGAGAAGGACTGGACTTAACACTTC-3'

Other clones with same target sequence:

(none)