Construct: shRNA TRCN0000173470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133746.2-1769s1c1
DNA Barcode:
GCTCACAGCTATCAGAAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Calhm2 (72691)
Gene Description:
calcium homeostasis modulator 2
Transcript:
RefSeq NM_133746.2 (NON-CURRENT)
Match location:
Position 1769 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACAGCTATCAGAAAGTA
Hairpin Sequence:
5'-CCGG-GCTCACAGCTATCAGAAAGTA-CTCGAG-TACTTTCTGATAGCTGTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACAGCTATCAGAAAGTACTCGAGTACTTTCTGATAGCTGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACAGCTATCAGAAAGTACTCGAGTACTTTCTGATAGCTGTGAGC-3'

Other clones with same target sequence:

(none)