Construct: shRNA TRCN0000173480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012520.1-133s1c1
DNA Barcode:
GCTATGTGAAGAGCAGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klri1 (503550)
Gene Description:
killer cell lectin-like receptor family I member 1
Transcript:
RefSeq NM_001012520.1 (NON-CURRENT)
Match location:
Position 133 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGTGAAGAGCAGCTAAA
Hairpin Sequence:
5'-CCGG-GCTATGTGAAGAGCAGCTAAA-CTCGAG-TTTAGCTGCTCTTCACATAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGTGAAGAGCAGCTAAACTCGAGTTTAGCTGCTCTTCACATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGTGAAGAGCAGCTAAACTCGAGTTTAGCTGCTCTTCACATAGC-3'

Other clones with same target sequence:

  1. TRCN0000254874