Construct: shRNA TRCN0000173511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029624.2-1196s1c1
DNA Barcode:
GCAGATCATGAACACCTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lmf1 (76483)
Gene Description:
lipase maturation factor 1
Transcript:
RefSeq NM_029624.2 (NON-CURRENT)
Match location:
Position 1196 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATCATGAACACCTCCTT
Hairpin Sequence:
5'-CCGG-GCAGATCATGAACACCTCCTT-CTCGAG-AAGGAGGTGTTCATGATCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATCATGAACACCTCCTTCTCGAGAAGGAGGTGTTCATGATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATCATGAACACCTCCTTCTCGAGAAGGAGGTGTTCATGATCTGC-3'

Other clones with same target sequence:

(none)