Construct: shRNA TRCN0000173575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026644.1-933s1c1
DNA Barcode:
GCTGGGAGTCTTAAATGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agpat4 (68262)
Gene Description:
1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
Transcript:
RefSeq NM_026644.1 (NON-CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGAGTCTTAAATGGAAA
Hairpin Sequence:
5'-CCGG-GCTGGGAGTCTTAAATGGAAA-CTCGAG-TTTCCATTTAAGACTCCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGAGTCTTAAATGGAAACTCGAGTTTCCATTTAAGACTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGAGTCTTAAATGGAAACTCGAGTTTCCATTTAAGACTCCCAGC-3'

Other clones with same target sequence:

(none)