Construct: shRNA TRCN0000173592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023060.2-2251s1c1
DNA Barcode:
GACCTTGAAGAATGGAGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eefsec (65967)
Gene Description:
eukaryotic elongation factor, selenocysteine-tRNA-specific
Transcript:
RefSeq NM_023060.2 (NON-CURRENT)
Match location:
Position 2251 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTTGAAGAATGGAGGTTT
Hairpin Sequence:
5'-CCGG-GACCTTGAAGAATGGAGGTTT-CTCGAG-AAACCTCCATTCTTCAAGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTTGAAGAATGGAGGTTTCTCGAGAAACCTCCATTCTTCAAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTTGAAGAATGGAGGTTTCTCGAGAAACCTCCATTCTTCAAGGTC-3'

Other clones with same target sequence:

  1. TRCN0000292827