Construct: shRNA TRCN0000173672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146114.1-880s1c1
DNA Barcode:
CCCTGTGGTATAACTTCCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dclre1c (227525)
Gene Description:
DNA cross-link repair 1C
Transcript:
RefSeq NM_146114.1 (NON-CURRENT)
Match location:
Position 880 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTGGTATAACTTCCCAA
Hairpin Sequence:
5'-CCGG-CCCTGTGGTATAACTTCCCAA-CTCGAG-TTGGGAAGTTATACCACAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTGGTATAACTTCCCAACTCGAGTTGGGAAGTTATACCACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTGGTATAACTTCCCAACTCGAGTTGGGAAGTTATACCACAGGG-3'

Other clones with same target sequence:

(none)