Construct: shRNA TRCN0000173754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024281.1-614s1c1
DNA Barcode:
CCCTAAGGACAGAAAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rrbp1 (81910)
Gene Description:
ribosome binding protein 1
Transcript:
RefSeq NM_024281.1 (NON-CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAAGGACAGAAAGAAGAA
Hairpin Sequence:
5'-CCGG-CCCTAAGGACAGAAAGAAGAA-CTCGAG-TTCTTCTTTCTGTCCTTAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAAGGACAGAAAGAAGAACTCGAGTTCTTCTTTCTGTCCTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAAGGACAGAAAGAAGAACTCGAGTTCTTCTTTCTGTCCTTAGGG-3'

Other clones with same target sequence:

(none)