Construct: shRNA TRCN0000173778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019587.1-5711s1c1
DNA Barcode:
CCTGAGACCTGCCAACTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxnb3 (140571)
Gene Description:
plexin B3
Transcript:
RefSeq NM_019587.1 (NON-CURRENT)
Match location:
Position 5711 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGACCTGCCAACTTATA
Hairpin Sequence:
5'-CCGG-CCTGAGACCTGCCAACTTATA-CTCGAG-TATAAGTTGGCAGGTCTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGACCTGCCAACTTATACTCGAGTATAAGTTGGCAGGTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGACCTGCCAACTTATACTCGAGTATAAGTTGGCAGGTCTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000250626