Construct: shRNA TRCN0000173854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175475.2-1813s1c1
DNA Barcode:
CATGTTGAGTGCTACGGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp26b1 (232174)
Gene Description:
cytochrome P450, family 26, subfamily b, polypeptide 1
Transcript:
RefSeq NM_175475.2 (NON-CURRENT)
Match location:
Position 1813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTTGAGTGCTACGGTGTA
Hairpin Sequence:
5'-CCGG-CATGTTGAGTGCTACGGTGTA-CTCGAG-TACACCGTAGCACTCAACATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTTGAGTGCTACGGTGTACTCGAGTACACCGTAGCACTCAACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTTGAGTGCTACGGTGTACTCGAGTACACCGTAGCACTCAACATG-3'

Other clones with same target sequence:

(none)