Construct: shRNA TRCN0000173869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029936.1-1887s1c1
DNA Barcode:
GCGCCGTCAAGTTACACAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Specc1 (432572)
Gene Description:
sperm antigen with calponin homology and coiled-coil domains 1
Transcript:
RefSeq NM_001029936.1 (NON-CURRENT)
Match location:
Position 1887 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCGTCAAGTTACACAATA
Hairpin Sequence:
5'-CCGG-GCGCCGTCAAGTTACACAATA-CTCGAG-TATTGTGTAACTTGACGGCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCGTCAAGTTACACAATACTCGAGTATTGTGTAACTTGACGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCGTCAAGTTACACAATACTCGAGTATTGTGTAACTTGACGGCGC-3'

Other clones with same target sequence:

  1. TRCN0000347254