Construct: shRNA TRCN0000173960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133942.1-533s1c1
DNA Barcode:
GCTGGGTATTGTGTGAAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekha1 (101476)
Gene Description:
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Transcript:
RefSeq NM_133942.1 (NON-CURRENT)
Match location:
Position 533 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGTATTGTGTGAAGCAA
Hairpin Sequence:
5'-CCGG-GCTGGGTATTGTGTGAAGCAA-CTCGAG-TTGCTTCACACAATACCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGTATTGTGTGAAGCAACTCGAGTTGCTTCACACAATACCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGTATTGTGTGAAGCAACTCGAGTTGCTTCACACAATACCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000320317