Construct: shRNA TRCN0000173974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178678.2-1007s1c1
DNA Barcode:
GCTTGAGGAAGCTGCTTAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrtm3 (216028)
Gene Description:
leucine rich repeat transmembrane neuronal 3
Transcript:
RefSeq NM_178678.2 (NON-CURRENT)
Match location:
Position 1007 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAGGAAGCTGCTTAGTT
Hairpin Sequence:
5'-CCGG-GCTTGAGGAAGCTGCTTAGTT-CTCGAG-AACTAAGCAGCTTCCTCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAGGAAGCTGCTTAGTTCTCGAGAACTAAGCAGCTTCCTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAGGAAGCTGCTTAGTTCTCGAGAACTAAGCAGCTTCCTCAAGC-3'

Other clones with same target sequence:

(none)