Construct: shRNA TRCN0000174026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009431.1-3235s1c1
DNA Barcode:
CCAGGAATAGCAACAGCGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctr9 (22083)
Gene Description:
CTR9 homolog, Paf1/RNA polymerase II complex component
Transcript:
RefSeq NM_009431.1 (NON-CURRENT)
Match location:
Position 3235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAATAGCAACAGCGATT
Hairpin Sequence:
5'-CCGG-CCAGGAATAGCAACAGCGATT-CTCGAG-AATCGCTGTTGCTATTCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAATAGCAACAGCGATTCTCGAGAATCGCTGTTGCTATTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAATAGCAACAGCGATTCTCGAGAATCGCTGTTGCTATTCCTGG-3'

Other clones with same target sequence:

(none)