Construct: shRNA TRCN0000174046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146012.1-500s1c1
DNA Barcode:
CCCTGCATCTTACATCTTCCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctdsp2 (52468)
Gene Description:
CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
Transcript:
RefSeq NM_146012.1 (NON-CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCATCTTACATCTTCCA
Hairpin Sequence:
5'-CCGG-CCCTGCATCTTACATCTTCCA-CTCGAG-TGGAAGATGTAAGATGCAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCATCTTACATCTTCCACTCGAGTGGAAGATGTAAGATGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCATCTTACATCTTCCACTCGAGTGGAAGATGTAAGATGCAGGG-3'

Other clones with same target sequence:

(none)