Construct: shRNA TRCN0000174066

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133197.1-2769s1c2
DNA Barcode:
GCAGCTTGGAATGGGATGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcf2 (109904)
Gene Description:
mcf.2 transforming sequence
Transcript:
RefSeq NM_133197.1 (NON-CURRENT)
Match location:
Position 2769 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174066)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTTGGAATGGGATGTTA
Hairpin Sequence:
5'-CCGG-GCAGCTTGGAATGGGATGTTA-CTCGAG-TAACATCCCATTCCAAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTTGGAATGGGATGTTACTCGAGTAACATCCCATTCCAAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTTGGAATGGGATGTTACTCGAGTAACATCCCATTCCAAGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000042656