Construct: shRNA TRCN0000174079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001567.1-786s1c2
DNA Barcode:
CGTCCACGACAACTACAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE9A (5152)
Gene Description:
phosphodiesterase 9A
Transcript:
RefSeq NM_001001567.1 (CURRENT)
Match location:
Position 786 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCCACGACAACTACAGAAA
Hairpin Sequence:
5'-CCGG-CGTCCACGACAACTACAGAAA-CTCGAG-TTTCTGTAGTTGTCGTGGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCCACGACAACTACAGAAACTCGAGTTTCTGTAGTTGTCGTGGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCCACGACAACTACAGAAACTCGAGTTTCTGTAGTTGTCGTGGACG-3'

Other clones with same target sequence:

  1. TRCN0000048896