Construct: shRNA TRCN0000174092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177966.3-1263s1c2
DNA Barcode:
CGAAAGTCTAAGTTCAGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE12 (201626)
Gene Description:
phosphodiesterase 12
Transcript:
RefSeq NM_177966.3 (NON-CURRENT)
Match location:
Position 1263 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGTCTAAGTTCAGCCTT
Hairpin Sequence:
5'-CCGG-CGAAAGTCTAAGTTCAGCCTT-CTCGAG-AAGGCTGAACTTAGACTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGTCTAAGTTCAGCCTTCTCGAGAAGGCTGAACTTAGACTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGTCTAAGTTCAGCCTTCTCGAGAAGGCTGAACTTAGACTTTCG-3'

Other clones with same target sequence:

  1. TRCN0000048984