Construct: shRNA TRCN0000174162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016580.2-1959s1c2
DNA Barcode:
GCACTGGAAATCCAAGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDH12 (51294)
Gene Description:
protocadherin 12
Transcript:
RefSeq NM_016580.2 (NON-CURRENT)
Match location:
Position 1959 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGGAAATCCAAGAAGAT
Hairpin Sequence:
5'-CCGG-GCACTGGAAATCCAAGAAGAT-CTCGAG-ATCTTCTTGGATTTCCAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGGAAATCCAAGAAGATCTCGAGATCTTCTTGGATTTCCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGGAAATCCAAGAAGATCTCGAGATCTTCTTGGATTTCCAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000053914