Construct: shRNA TRCN0000174208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005941.2-143s1c2
DNA Barcode:
CGGTTGGATTTCGTGCATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MMP16 (4325)
Gene Description:
matrix metallopeptidase 16
Transcript:
RefSeq NM_005941.2 (NON-CURRENT)
Match location:
Position 143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTGGATTTCGTGCATCAT
Hairpin Sequence:
5'-CCGG-CGGTTGGATTTCGTGCATCAT-CTCGAG-ATGATGCACGAAATCCAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTGGATTTCGTGCATCATCTCGAGATGATGCACGAAATCCAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTGGATTTCGTGCATCATCTCGAGATGATGCACGAAATCCAACCG-3'

Other clones with same target sequence:

  1. TRCN0000052248