Construct: shRNA TRCN0000174241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145830.1-272s1c2
DNA Barcode:
CCGAGAGAGTTCATAGCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehmt2 (110147)
Gene Description:
euchromatic histone lysine N-methyltransferase 2
Transcript:
RefSeq NM_145830.1 (NON-CURRENT)
Match location:
Position 272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGAGAGTTCATAGCTCTT
Hairpin Sequence:
5'-CCGG-CCGAGAGAGTTCATAGCTCTT-CTCGAG-AAGAGCTATGAACTCTCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGAGAGTTCATAGCTCTTCTCGAGAAGAGCTATGAACTCTCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGAGAGTTCATAGCTCTTCTCGAGAAGAGCTATGAACTCTCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000054545
  2. TRCN0000301235