Construct: shRNA TRCN0000174248
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_059399.4-101s1c2
- DNA Barcode:
- CAGAAATGAAATTCTGTGCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CIB4 (130106)
- Gene Description:
- calcium and integrin binding family member 4
- Transcript:
- RefSeq XM_059399.4 (NON-CURRENT)
- Match location:
- Position 101 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000174248)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGAAATGAAATTCTGTGCAT
- Hairpin Sequence:
-
5'-CCGG-CAGAAATGAAATTCTGTGCAT-CTCGAG-ATGCACAGAATTTCATTTCTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGAAATGAAATTCTGTGCATCTCGAGATGCACAGAATTTCATTTCTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGAAATGAAATTCTGTGCATCTCGAGATGCACAGAATTTCATTTCTG-3'
Other clones with same target sequence:
-
TRCN0000052671
