Construct: shRNA TRCN0000174256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_374879.1-547s1c2
DNA Barcode:
GCTGGATGTTCTTAAAGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPMT1 (114971)
Gene Description:
protein tyrosine phosphatase mitochondrial 1
Transcript:
RefSeq XM_374879.1 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATGTTCTTAAAGAGTT
Hairpin Sequence:
5'-CCGG-GCTGGATGTTCTTAAAGAGTT-CTCGAG-AACTCTTTAAGAACATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATGTTCTTAAAGAGTTCTCGAGAACTCTTTAAGAACATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATGTTCTTAAAGAGTTCTCGAGAACTCTTTAAGAACATCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000052683
  2. TRCN0000289105