Construct: shRNA TRCN0000174272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033616.2-2151s1c1
DNA Barcode:
CCTGTTTAGAAACTGTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csprs (114564)
Gene Description:
component of Sp100-rs
Transcript:
RefSeq NM_033616.2 (NON-CURRENT)
Match location:
Position 2151 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTTAGAAACTGTTCTTT
Hairpin Sequence:
5'-CCGG-CCTGTTTAGAAACTGTTCTTT-CTCGAG-AAAGAACAGTTTCTAAACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTTAGAAACTGTTCTTTCTCGAGAAAGAACAGTTTCTAAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTTAGAAACTGTTCTTTCTCGAGAAAGAACAGTTTCTAAACAGG-3'

Other clones with same target sequence:

(none)