Construct: shRNA TRCN0000174296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025793.1-1933s1c1
DNA Barcode:
CCATAGGAACACACTAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr45b (66840)
Gene Description:
WD repeat domain 45B
Transcript:
RefSeq NM_025793.1 (NON-CURRENT)
Match location:
Position 1933 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAGGAACACACTAACATT
Hairpin Sequence:
5'-CCGG-CCATAGGAACACACTAACATT-CTCGAG-AATGTTAGTGTGTTCCTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAGGAACACACTAACATTCTCGAGAATGTTAGTGTGTTCCTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAGGAACACACTAACATTCTCGAGAATGTTAGTGTGTTCCTATGG-3'

Other clones with same target sequence:

  1. TRCN0000292934