Construct: shRNA TRCN0000174298

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027992.1-1447s1c1
DNA Barcode:
CCTATTATTCATGGAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem106b (71900)
Gene Description:
transmembrane protein 106B
Transcript:
RefSeq NM_027992.1 (NON-CURRENT)
Match location:
Position 1447 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174298)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATTATTCATGGAGAACTT
Hairpin Sequence:
5'-CCGG-CCTATTATTCATGGAGAACTT-CTCGAG-AAGTTCTCCATGAATAATAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATTATTCATGGAGAACTTCTCGAGAAGTTCTCCATGAATAATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATTATTCATGGAGAACTTCTCGAGAAGTTCTCCATGAATAATAGG-3'

Other clones with same target sequence:

  1. TRCN0000345668