Construct: shRNA TRCN0000174355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181074.4-2547s1c1
DNA Barcode:
CGGCAGAGTCAATAATTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lingo1 (235402)
Gene Description:
leucine rich repeat and Ig domain containing 1
Transcript:
RefSeq NM_181074.4 (CURRENT)
Match location:
Position 2547 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAGAGTCAATAATTCAAT
Hairpin Sequence:
5'-CCGG-CGGCAGAGTCAATAATTCAAT-CTCGAG-ATTGAATTATTGACTCTGCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAGAGTCAATAATTCAATCTCGAGATTGAATTATTGACTCTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAGAGTCAATAATTCAATCTCGAGATTGAATTATTGACTCTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000278640