Construct: shRNA TRCN0000174393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019867.1-960s1c1
DNA Barcode:
CTATATCACTTACGTCAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ngef (53972)
Gene Description:
neuronal guanine nucleotide exchange factor
Transcript:
RefSeq NM_019867.1 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATATCACTTACGTCAGTAA
Hairpin Sequence:
5'-CCGG-CTATATCACTTACGTCAGTAA-CTCGAG-TTACTGACGTAAGTGATATAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATATCACTTACGTCAGTAACTCGAGTTACTGACGTAAGTGATATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATATCACTTACGTCAGTAACTCGAGTTACTGACGTAAGTGATATAG-3'

Other clones with same target sequence:

(none)