Construct: shRNA TRCN0000174404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153055.2-2068s1c1
DNA Barcode:
GAAGAGGTAGAACTGAAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec63 (140740)
Gene Description:
SEC63-like (S. cerevisiae)
Transcript:
RefSeq NM_153055.2 (NON-CURRENT)
Match location:
Position 2068 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGGTAGAACTGAAGTTT
Hairpin Sequence:
5'-CCGG-GAAGAGGTAGAACTGAAGTTT-CTCGAG-AAACTTCAGTTCTACCTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGGTAGAACTGAAGTTTCTCGAGAAACTTCAGTTCTACCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGGTAGAACTGAAGTTTCTCGAGAAACTTCAGTTCTACCTCTTC-3'

Other clones with same target sequence:

(none)