Construct: shRNA TRCN0000174423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026333.2-655s1c1
DNA Barcode:
CCTTACAACTATCCATATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2010106E10Rik (67715)
Gene Description:
RIKEN cDNA 2010106E10 gene
Transcript:
RefSeq NM_026333.2 (NON-CURRENT)
Match location:
Position 655 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTACAACTATCCATATCTT
Hairpin Sequence:
5'-CCGG-CCTTACAACTATCCATATCTT-CTCGAG-AAGATATGGATAGTTGTAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTACAACTATCCATATCTTCTCGAGAAGATATGGATAGTTGTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTACAACTATCCATATCTTCTCGAGAAGATATGGATAGTTGTAAGG-3'

Other clones with same target sequence:

(none)