Construct: shRNA TRCN0000174454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025779.2-566s1c1
DNA Barcode:
CATCATTAACAAACTACGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcub (66815)
Gene Description:
mitochondrial calcium uniporter dominant negative beta subunit
Transcript:
RefSeq NM_025779.2 (NON-CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCATTAACAAACTACGGTA
Hairpin Sequence:
5'-CCGG-CATCATTAACAAACTACGGTA-CTCGAG-TACCGTAGTTTGTTAATGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCATTAACAAACTACGGTACTCGAGTACCGTAGTTTGTTAATGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCATTAACAAACTACGGTACTCGAGTACCGTAGTTTGTTAATGATG-3'

Other clones with same target sequence:

(none)