Construct: shRNA TRCN0000174457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026142.1-1867s1c1
DNA Barcode:
CAACTTGAATCTGAAGAGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
3632451O06Rik (67419)
Gene Description:
RIKEN cDNA 3632451O06 gene
Transcript:
RefSeq NM_026142.1 (NON-CURRENT)
Match location:
Position 1867 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTTGAATCTGAAGAGGTA
Hairpin Sequence:
5'-CCGG-CAACTTGAATCTGAAGAGGTA-CTCGAG-TACCTCTTCAGATTCAAGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTTGAATCTGAAGAGGTACTCGAGTACCTCTTCAGATTCAAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTTGAATCTGAAGAGGTACTCGAGTACCTCTTCAGATTCAAGTTG-3'

Other clones with same target sequence:

(none)