Construct: shRNA TRCN0000174487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026713.1-450s1c1
DNA Barcode:
CTCAGACTTCAAGAAGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mogat1 (68393)
Gene Description:
monoacylglycerol O-acyltransferase 1
Transcript:
RefSeq NM_026713.1 (NON-CURRENT)
Match location:
Position 450 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGACTTCAAGAAGCTATT
Hairpin Sequence:
5'-CCGG-CTCAGACTTCAAGAAGCTATT-CTCGAG-AATAGCTTCTTGAAGTCTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGACTTCAAGAAGCTATTCTCGAGAATAGCTTCTTGAAGTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGACTTCAAGAAGCTATTCTCGAGAATAGCTTCTTGAAGTCTGAG-3'

Other clones with same target sequence:

(none)