Construct: shRNA TRCN0000174516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029561.1-759s1c1
DNA Barcode:
CTTTGGTGGATATTTCTCGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndfip2 (76273)
Gene Description:
Nedd4 family interacting protein 2
Transcript:
RefSeq NM_029561.1 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGTGGATATTTCTCGTA
Hairpin Sequence:
5'-CCGG-CTTTGGTGGATATTTCTCGTA-CTCGAG-TACGAGAAATATCCACCAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGTGGATATTTCTCGTACTCGAGTACGAGAAATATCCACCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGTGGATATTTCTCGTACTCGAGTACGAGAAATATCCACCAAAG-3'

Other clones with same target sequence:

  1. TRCN0000278733