Construct: shRNA TRCN0000174523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172381.2-760s1c1
DNA Barcode:
CCTTACGGATATGTGTTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
AI314180 (230249)
Gene Description:
expressed sequence AI314180
Transcript:
RefSeq NM_172381.2 (CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTACGGATATGTGTTAAAT
Hairpin Sequence:
5'-CCGG-CCTTACGGATATGTGTTAAAT-CTCGAG-ATTTAACACATATCCGTAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTACGGATATGTGTTAAATCTCGAGATTTAACACATATCCGTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTACGGATATGTGTTAAATCTCGAGATTTAACACATATCCGTAAGG-3'

Other clones with same target sequence:

  1. TRCN0000278797