Construct: shRNA TRCN0000174524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172475.2-482s1c1
DNA Barcode:
CTTCTGTGTCAGATTCTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frmd4a (209630)
Gene Description:
FERM domain containing 4A
Transcript:
RefSeq NM_172475.2 (NON-CURRENT)
Match location:
Position 482 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTGTGTCAGATTCTACAT
Hairpin Sequence:
5'-CCGG-CTTCTGTGTCAGATTCTACAT-CTCGAG-ATGTAGAATCTGACACAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTGTGTCAGATTCTACATCTCGAGATGTAGAATCTGACACAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTGTGTCAGATTCTACATCTCGAGATGTAGAATCTGACACAGAAG-3'

Other clones with same target sequence:

(none)