Construct: shRNA TRCN0000174613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005507.1-4267s1c1
DNA Barcode:
GCTGTACGATTTCAAATTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smg7 (226517)
Gene Description:
Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Transcript:
RefSeq NM_001005507.1 (NON-CURRENT)
Match location:
Position 4267 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTACGATTTCAAATTCAT
Hairpin Sequence:
5'-CCGG-GCTGTACGATTTCAAATTCAT-CTCGAG-ATGAATTTGAAATCGTACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTACGATTTCAAATTCATCTCGAGATGAATTTGAAATCGTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTACGATTTCAAATTCATCTCGAGATGAATTTGAAATCGTACAGC-3'

Other clones with same target sequence:

(none)