Construct: shRNA TRCN0000174624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013747.2-3199s1c1
DNA Barcode:
GCAGACAGTAATTTGACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Golga5 (27277)
Gene Description:
golgi autoantigen, golgin subfamily a, 5
Transcript:
RefSeq NM_013747.2 (NON-CURRENT)
Match location:
Position 3199 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACAGTAATTTGACAAAT
Hairpin Sequence:
5'-CCGG-GCAGACAGTAATTTGACAAAT-CTCGAG-ATTTGTCAAATTACTGTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACAGTAATTTGACAAATCTCGAGATTTGTCAAATTACTGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACAGTAATTTGACAAATCTCGAGATTTGTCAAATTACTGTCTGC-3'

Other clones with same target sequence:

(none)