Construct: shRNA TRCN0000174642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144536.1-939s1c1
DNA Barcode:
GCATGACAAATCCACCATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkal1 (68916)
Gene Description:
CDK5 regulatory subunit associated protein 1-like 1
Transcript:
RefSeq NM_144536.1 (NON-CURRENT)
Match location:
Position 939 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGACAAATCCACCATATA
Hairpin Sequence:
5'-CCGG-GCATGACAAATCCACCATATA-CTCGAG-TATATGGTGGATTTGTCATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGACAAATCCACCATATACTCGAGTATATGGTGGATTTGTCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGACAAATCCACCATATACTCGAGTATATGGTGGATTTGTCATGC-3'

Other clones with same target sequence:

(none)