Construct: shRNA TRCN0000174654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_212450.1-811s1c1
DNA Barcode:
GCCATCACTAAATAATGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctdspl2 (329506)
Gene Description:
CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
Transcript:
RefSeq NM_212450.1 (NON-CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCACTAAATAATGGTTT
Hairpin Sequence:
5'-CCGG-GCCATCACTAAATAATGGTTT-CTCGAG-AAACCATTATTTAGTGATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCACTAAATAATGGTTTCTCGAGAAACCATTATTTAGTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCACTAAATAATGGTTTCTCGAGAAACCATTATTTAGTGATGGC-3'

Other clones with same target sequence:

(none)