Construct: shRNA TRCN0000174674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172861.2-2204s1c1
DNA Barcode:
GTGAATATCTATCTCATGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc7a14 (241919)
Gene Description:
solute carrier family 7 (cationic amino acid transporter, y+ system), member 14
Transcript:
RefSeq NM_172861.2 (NON-CURRENT)
Match location:
Position 2204 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAATATCTATCTCATGCTA
Hairpin Sequence:
5'-CCGG-GTGAATATCTATCTCATGCTA-CTCGAG-TAGCATGAGATAGATATTCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAATATCTATCTCATGCTACTCGAGTAGCATGAGATAGATATTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAATATCTATCTCATGCTACTCGAGTAGCATGAGATAGATATTCAC-3'

Other clones with same target sequence:

(none)