Construct: shRNA TRCN0000174688
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_018887.2-429s1c1
- DNA Barcode:
- GAAGAAGGAATCAATGTGCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cyp39a1 (56050)
- Gene Description:
- cytochrome P450, family 39, subfamily a, polypeptide 1
- Transcript:
- RefSeq NM_018887.2 (NON-CURRENT)
- Match location:
- Position 429 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000174688)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGAAGGAATCAATGTGCTT
- Hairpin Sequence:
-
5'-CCGG-GAAGAAGGAATCAATGTGCTT-CTCGAG-AAGCACATTGATTCCTTCTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGAAGGAATCAATGTGCTTCTCGAGAAGCACATTGATTCCTTCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGAAGGAATCAATGTGCTTCTCGAGAAGCACATTGATTCCTTCTTC-3'
Other clones with same target sequence:
-
TRCN0000329412
