Construct: shRNA TRCN0000174706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144881.2-1874s1c1
DNA Barcode:
GCTTCATCAATCACAGTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hhat (226861)
Gene Description:
hedgehog acyltransferase
Transcript:
RefSeq NM_144881.2 (NON-CURRENT)
Match location:
Position 1874 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCATCAATCACAGTAAAT
Hairpin Sequence:
5'-CCGG-GCTTCATCAATCACAGTAAAT-CTCGAG-ATTTACTGTGATTGATGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCATCAATCACAGTAAATCTCGAGATTTACTGTGATTGATGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCATCAATCACAGTAAATCTCGAGATTTACTGTGATTGATGAAGC-3'

Other clones with same target sequence:

(none)