Construct: shRNA TRCN0000174726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026432.2-1177s1c1
DNA Barcode:
GCAAAGTTTCTGATTTGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Saraf (67887)
Gene Description:
store-operated calcium entry-associated regulatory factor
Transcript:
RefSeq NM_026432.2 (NON-CURRENT)
Match location:
Position 1177 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGTTTCTGATTTGTCAT
Hairpin Sequence:
5'-CCGG-GCAAAGTTTCTGATTTGTCAT-CTCGAG-ATGACAAATCAGAAACTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGTTTCTGATTTGTCATCTCGAGATGACAAATCAGAAACTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGTTTCTGATTTGTCATCTCGAGATGACAAATCAGAAACTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000340640