Construct: shRNA TRCN0000174761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138676.1-113s1c1
DNA Barcode:
GAAGTGATTCATCTGAACGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shkbp1 (192192)
Gene Description:
Sh3kbp1 binding protein 1
Transcript:
RefSeq NM_138676.1 (NON-CURRENT)
Match location:
Position 113 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGATTCATCTGAACGTA
Hairpin Sequence:
5'-CCGG-GAAGTGATTCATCTGAACGTA-CTCGAG-TACGTTCAGATGAATCACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGATTCATCTGAACGTACTCGAGTACGTTCAGATGAATCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGATTCATCTGAACGTACTCGAGTACGTTCAGATGAATCACTTC-3'

Other clones with same target sequence:

  1. TRCN0000319969