Construct: shRNA TRCN0000174794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010267.2-343s1c1
DNA Barcode:
GCCACTCAGATCATTGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gdap1 (14545)
Gene Description:
ganglioside-induced differentiation-associated-protein 1
Transcript:
RefSeq NM_010267.2 (NON-CURRENT)
Match location:
Position 343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTCAGATCATTGATTAT
Hairpin Sequence:
5'-CCGG-GCCACTCAGATCATTGATTAT-CTCGAG-ATAATCAATGATCTGAGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTCAGATCATTGATTATCTCGAGATAATCAATGATCTGAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTCAGATCATTGATTATCTCGAGATAATCAATGATCTGAGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000118969