Construct: shRNA TRCN0000174885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019432.2-696s1c1
DNA Barcode:
GAGATTGAATAGGACAACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem37 (170706)
Gene Description:
transmembrane protein 37
Transcript:
RefSeq NM_019432.2 (CURRENT)
Match location:
Position 696 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATTGAATAGGACAACCAA
Hairpin Sequence:
5'-CCGG-GAGATTGAATAGGACAACCAA-CTCGAG-TTGGTTGTCCTATTCAATCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATTGAATAGGACAACCAACTCGAGTTGGTTGTCCTATTCAATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATTGAATAGGACAACCAACTCGAGTTGGTTGTCCTATTCAATCTC-3'

Other clones with same target sequence:

(none)