Construct: shRNA TRCN0000174897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080468.1-1006s1c1
DNA Barcode:
GCCTAGAAATCAAATTGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rxfp2 (140498)
Gene Description:
relaxin/insulin-like family peptide receptor 2
Transcript:
RefSeq NM_080468.1 (NON-CURRENT)
Match location:
Position 1006 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGAAATCAAATTGGTTT
Hairpin Sequence:
5'-CCGG-GCCTAGAAATCAAATTGGTTT-CTCGAG-AAACCAATTTGATTTCTAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGAAATCAAATTGGTTTCTCGAGAAACCAATTTGATTTCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGAAATCAAATTGGTTTCTCGAGAAACCAATTTGATTTCTAGGC-3'

Other clones with same target sequence:

(none)