Construct: shRNA TRCN0000174920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030736.1-1853s1c1
DNA Barcode:
GACAATAACACTGACTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r148 (81011)
Gene Description:
vomeronasal 1 receptor 148
Transcript:
RefSeq NM_030736.1 (NON-CURRENT)
Match location:
Position 1853 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAATAACACTGACTCTAAA
Hairpin Sequence:
5'-CCGG-GACAATAACACTGACTCTAAA-CTCGAG-TTTAGAGTCAGTGTTATTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAATAACACTGACTCTAAACTCGAGTTTAGAGTCAGTGTTATTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAATAACACTGACTCTAAACTCGAGTTTAGAGTCAGTGTTATTGTC-3'

Other clones with same target sequence:

(none)